Daniel A. Fletcher

Purnendu Chatterjee Chair in Engineering Biological Systems, Bioengineering Faculty Scientist, Lawrence Berkeley National Laboratory
Office Address: 708A Stanley Hall
Email: fletch@berkeley.edu
For more information, see: https://fletchlab.berkeley.edu/
Office hours: 708A Stanley
Research areas:
Bioinstrumentation, Cell and Tissue Engineering
The Fletcher Lab develops diagnostic technologies and studies mechanical regulation of membrane and cytoskeleton organization in the context of cell motility, signaling, and host-pathogen interactions. We specialize in development of optical microscopy, force microscopy, and microfluidic technologies to understand fundamental organizational principles through both in vitro reconstitution and live cell experiments. Recent work includes investigating the mechano-biochemistry of branched actin network assembly with force microscopy, studying membrane deformation by protein crowding and oligomerization with model membranes, and reconstituting spindle scaling in encapsulated cytoplasmic extracts. The long-term goal of our work is to understand and harness spatial organization for therapeutic applications in cancer and infectious diseases.
Education
- 2002 Postdoctoral Fellow, Stanford University, Biochemistry
- 2001 Ph.D., Stanford University, Mechanical Engineering
- 1997 D.Phil., Oxford University, Engineering Science
- 1994 B.S.E., Princeton University, Mechanical & Aerospace Engineering
Experience & Training
- 2015-2019 Chair, Department of Bioengineering, University of California, Berkeley
- 2009-2015 Associate Chair, Department of Bioengineering, UC Berkeley
- 2010-present Professor, Bioengineering, UC Berkeley
- 2007-2015 Deputy Division Director, Physical Biosciences, Lawrence Berkeley National Lab
- 2007-2010 Associate Professor, Bioengineering, UC Berkeley
- 2002-2007 Assistant Professor, Bioengineering, UC Berkeley
- 2003-present Faculty Scientist, Lawrence Berkeley National Laboratory
- 2003-present Member, Nanoscale Science & Engineering Graduate Group, UC Berkeley
- 2002-present Member, Bioengineering Graduate Group, UC Berkeley & UCSF
- 2002-present Member, Biophysics Graduate Group, UC Berkeley
- 2002-present Faculty Affiliate, QB3, UC Berkeley
Honors & Awards
- 2017-2021 Chan Zuckerberg Biohub Investigator
- 2013-present Purnendu Chatterjee Endowed Chair in Engineering Biological Systems, UC Berkeley
- 2010-2013 Lester John and Lynne Dewar Lloyd Distinguished Professorship, UC Berkeley
- 2010 Bioengineering Teaching Award, Outstanding Instructor, Bioengineering Honor Society, UC Berkeley
- 2010 Tech Award Laureate, Museum of Technology and Innovation, San Jose, CA
- 2009 Bill and Melinda Gates Foundation Grand Challenges Exploration Grant
- 2008-2009 White House Fellow, Washington, DC
- 2005 Hellman Faculty Fund Award, UC Berkeley
- 2004 National Science Foundation CAREER Award
- 2003 UC Berkeley Presidential Chair Fellow
- 2001 National Inventors Hall of Fame Collegiate Inventors Award
- 1996 National Science Foundation Graduate Research Fellowship
- 1994 Rhodes Scholarship
2024
Coulibaly, Jean T.; Silue, Kigbafori D.; de Leon Derby, Maria Diaz; Fletcher, Daniel A.; Fisher, Karla N.; Andrews, Jason R.; Bogoch, Isaac I.
In: AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, vol. 111, no. 2, pp. 387-390, 2024, ISSN: 0002-9637.
@article{WOS:001299762300025,
title = {Rapid and Comprehensive Screening for Urogenital and Gastrointestinal
Schistosomiasis with Handheld Digital Microscopy Combined with
Circulating Cathodic Antigen Testing},
author = {Jean T. Coulibaly and Kigbafori D. Silue and Maria Diaz de Leon Derby and Daniel A. Fletcher and Karla N. Fisher and Jason R. Andrews and Isaac I. Bogoch},
doi = {10.4269/ajtmh.24-0043},
issn = {0002-9637},
year = {2024},
date = {2024-08-01},
journal = {AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE},
volume = {111},
number = {2},
pages = {387-390},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Lang, Stuart; Fletcher, Daniel A.; Petit, Alain-Pierre; Luise, Nicola; Fyfe, Paul; Zuccotto, Fabio; Porter, David; Hope, Anthony; Bellany, Fiona; Kerr, Catrina; Mackenzie, Claire J.; Wyatt, Paul G.; Gray, David W.
Application of an NMR/Crystallography Fragment Screening Platform for the Assessment and Rapid Discovery of New HIV-CA Binding Fragments Journal Article
In: CHEMMEDCHEM, vol. 19, no. 13, 2024, ISSN: 1860-7179.
@article{WOS:001214310500001,
title = {Application of an NMR/Crystallography Fragment Screening Platform for
the Assessment and Rapid Discovery of New HIV-CA Binding Fragments},
author = {Stuart Lang and Daniel A. Fletcher and Alain-Pierre Petit and Nicola Luise and Paul Fyfe and Fabio Zuccotto and David Porter and Anthony Hope and Fiona Bellany and Catrina Kerr and Claire J. Mackenzie and Paul G. Wyatt and David W. Gray},
doi = {10.1002/cmdc.202400025},
issn = {1860-7179},
year = {2024},
date = {2024-07-01},
journal = {CHEMMEDCHEM},
volume = {19},
number = {13},
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pubstate = {published},
tppubtype = {article}
}
2023
Takatori, Sho C.; Son, Sungmin; Lee, Daniel S. W.; Fletcher, Daniel A.
Engineered molecular sensors for quantifying cell surface crowding Journal Article
In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 120, no. 21, 2023, ISSN: 0027-8424.
@article{WOS:001039552600008,
title = {Engineered molecular sensors for quantifying cell surface crowding},
author = {Sho C. Takatori and Sungmin Son and Daniel S. W. Lee and Daniel A. Fletcher},
doi = {10.1073/pnas.2219778120},
issn = {0027-8424},
year = {2023},
date = {2023-05-01},
journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF
AMERICA},
volume = {120},
number = {21},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Stevens, Adam J.; Harris, Andrew R.; Gerdts, Josiah; Kim, Ki H.; Trentesaux, Coralie; Ramirez, Jonathan T.; McKeithan, Wesley L.; Fattahi, Faranak; Klein, Ophir D.; Fletcher, Daniel A.; Lim, Wendell A.
Programming multicellular assembly with synthetic cell adhesion molecules Journal Article
In: NATURE, vol. 614, no. 7946, pp. 144+, 2023, ISSN: 0028-0836.
@article{WOS:000928188500007,
title = {Programming multicellular assembly with synthetic cell adhesion
molecules},
author = {Adam J. Stevens and Andrew R. Harris and Josiah Gerdts and Ki H. Kim and Coralie Trentesaux and Jonathan T. Ramirez and Wesley L. McKeithan and Faranak Fattahi and Ophir D. Klein and Daniel A. Fletcher and Wendell A. Lim},
doi = {10.1038/s41586-022-05622-z},
issn = {0028-0836},
year = {2023},
date = {2023-02-01},
journal = {NATURE},
volume = {614},
number = {7946},
pages = {144+},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Coulibaly, Jean T.; Silue, Kigbafori D.; Armstrong, Maxim; de Leon Derby, Maria Diaz; D'Ambrosio, Michael V.; Fletcher, Daniel A.; Keiser, Jennifer; Fisher, Karla; Andrews, Jason R.; Bogoch, Isaac I.
High Sensitivity of Mobile Phone Microscopy Screening for Schistosoma haematobium in Azaguie, Cote d'Ivoire Journal Article
In: AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, vol. 108, no. 1, pp. 41-43, 2023, ISSN: 0002-9637.
@article{WOS:000924080500011,
title = {High Sensitivity of Mobile Phone Microscopy Screening for \textit{Schistosoma
haematobium} in Azaguie, Cote d'Ivoire},
author = {Jean T. Coulibaly and Kigbafori D. Silue and Maxim Armstrong and Maria Diaz de Leon Derby and Michael V. D'Ambrosio and Daniel A. Fletcher and Jennifer Keiser and Karla Fisher and Jason R. Andrews and Isaac I. Bogoch},
doi = {10.4269/ajtmh.22-0527},
issn = {0002-9637},
year = {2023},
date = {2023-01-01},
journal = {AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE},
volume = {108},
number = {1},
pages = {41-43},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2022
Stevens, Adam J.; Harris, Andrew R.; Gerdts, Josiah; Kim, Ki H.; Trentesaux, Coralie; Ramirez, Jonathan T.; McKeithan, Wesley L.; Fattahi, Faranak; Klein, Ophir D.; Fletcher, Daniel A.; Lim, Wendell A.
Author Correction: Pan-cancer analysis of whole genomes Journal Article
In: NATURE, 2022, ISSN: 0028-0836.
@article{WOS:000920659000001,
title = {Author Correction: Pan-cancer analysis of whole genomes},
author = {Adam J. Stevens and Andrew R. Harris and Josiah Gerdts and Ki H. Kim and Coralie Trentesaux and Jonathan T. Ramirez and Wesley L. McKeithan and Faranak Fattahi and Ophir D. Klein and Daniel A. Fletcher and Wendell A. Lim},
doi = {10.1038/s41586-022-05598-w},
issn = {0028-0836},
year = {2022},
date = {2022-12-01},
journal = {NATURE},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Chandrasekaran, Sita S.; Agrawal, Shreeya; Fanton, Alison; Jangid, Aditya R.; Charrez, Berenice; Escajeda, Arturo M.; Son, Sungmin; Mcintosh, Roger; Tran, Huyen; Bhuiya, Abdul; de Leon Derby, Maria Diaz; Switz, Neil A.; Armstrong, Maxim; Harris, Andrew R.; Prywes, Noam; Lukarska, Maria; Biering, Scott B.; Smock, Dylan C. J.; Mok, Amanda; Knott, Gavin J.; Dang, Qi; Dis, Erik Van; Dugan, Eli; Kim, Shin; Liu, Tina Y.; Consortium, Igi Testing; Moehle, Erica A.; Kogut, Katherine; Eskenazi, Brenda; Harris, Eva; Stanley, Sarah A.; Lareau, Liana F.; Tan, Ming X.; Fletcher, Daniel A.; Doudna, Jennifer A.; Savage, David F.; Hsu, Patrick D.
Rapid detection of SARS-CoV-2 RNA in saliva via Cas13 Journal Article
In: NATURE BIOMEDICAL ENGINEERING, vol. 6, no. 8, pp. 944+, 2022, ISSN: 2157-846X.
@article{WOS:000839493700002,
title = {Rapid detection of SARS-CoV-2 RNA in saliva via Cas13},
author = {Sita S. Chandrasekaran and Shreeya Agrawal and Alison Fanton and Aditya R. Jangid and Berenice Charrez and Arturo M. Escajeda and Sungmin Son and Roger Mcintosh and Huyen Tran and Abdul Bhuiya and Maria Diaz de Leon Derby and Neil A. Switz and Maxim Armstrong and Andrew R. Harris and Noam Prywes and Maria Lukarska and Scott B. Biering and Dylan C. J. Smock and Amanda Mok and Gavin J. Knott and Qi Dang and Erik Van Dis and Eli Dugan and Shin Kim and Tina Y. Liu and Igi Testing Consortium and Erica A. Moehle and Katherine Kogut and Brenda Eskenazi and Eva Harris and Sarah A. Stanley and Liana F. Lareau and Ming X. Tan and Daniel A. Fletcher and Jennifer A. Doudna and David F. Savage and Patrick D. Hsu},
doi = {10.1038/s41551-022-00917-y},
issn = {2157-846X},
year = {2022},
date = {2022-08-01},
journal = {NATURE BIOMEDICAL ENGINEERING},
volume = {6},
number = {8},
pages = {944+},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Li, Tai-De; Bieling, Peter; Weichsel, Julian; Mullins, R. Dyche; Fletcher, Daniel A.
The molecular mechanism of load adaptation by branched actin networks Journal Article
In: ELIFE, vol. 11, 2022, ISSN: 2050-084X.
@article{WOS:000933871800001,
title = {The molecular mechanism of load adaptation by branched actin networks},
author = {Tai-De Li and Peter Bieling and Julian Weichsel and R. Dyche Mullins and Daniel A. Fletcher},
doi = {10.7554/eLife.73145},
issn = {2050-084X},
year = {2022},
date = {2022-06-01},
journal = {ELIFE},
volume = {11},
keywords = {},
pubstate = {published},
tppubtype = {article}
}