 | Gerard MarriottProfessor, Department of Bioengineering |
Research Interests
The Marriott laboratory seeks to understand the molecular and structural basis of complex cellular processes including motility and muscle contraction. These studies borrow concepts and principles from chemistry, biology, engineering and physics for the design and application of new optical probes and microscope imaging techniques that are used to investigate protein function and dynamics over a hierarchy of organizational levels, ranging from single molecules to cells within animals.
Education
1990~1992 Postdoc., Keio University, Physics
1987~1989 Postdoc., Max Planck Institute for Biophysical Chemistry, Biophysics
1987 Ph.D., University of Illinois, Biochemistry
1980 B.Sc. (Hons)., Birmingham University, UK, Biochemistry
Major Awards
Fluorescence Investigator Award. American Biophysics Society
Japan Society for the Promotion of Science Fellow
Top-ten Innovations of 2008 for OLID-FRET - The Scientist magazine
Alexander von Humboldt Society Fellow.
Professional Experience
1999-present Faculty Scientist, Computational and Theoretical Biology Department, Physical Biosciences Division, Lawrence Berkeley National Laboratory
Current Information:
2009-present: Professor, Department of Bioengineering, UC-Berkeley
2009-present Faculty Scientist, Physical Biosciences Division, Lawrence Berkeley National Laboratory
2005-2009 Professor, Department of Physiology, University of Wisconsin-Madison
2010-present Guest Professor. Tsinghua University Graduate School, Shenzhen, China
2006-present Guest Professor. Department of Bioengineering, SouthEast University, Nanjing, China
2005-2006 Visiting Professor, Department of Biochemistry, Stanford University
1999-2005 Associate Professor, Dept. of Physiology, University Wisconsin-Madison
1992-1999 C3 Professor (nachwuchsgruppenleiter), Max Planck Institute for Biochemistry, Martinsried, Germany
Selected Publications
1. Wu, L, Dai, YR and Marriott, G. (2011). Optical Control of Calcium Affinity in a Spiroamido-rhodamine Based Calcium Chelator, Chem. Letters. In press
2. Yan, Y., Marriott, M.E., Petchprayoon, C. and Marriott, G. (2011). Optical switches: High-contrast imaging in living cells and tissue. Biochemical Journal. 433 (411–422).
3. Yan, Y. and Marriott, G. (2011). Optical Manipulation of Protein Activity and Protein Interactions Using Caged Proteins and Optical Switch Protein Conjugates. In: Photo-control of Biological Function. Eds. Chambers, J. and Kramer, R. Elsevier Protocols. Humana Press. pp. 213-231
4. Petchprayoon, C. and Marriott, G. (2010). Synthesis and spectroscopic characterization of red-shifted spironaphthoxazine optical switch reagents for studies in living cell. Tetrahedron Letters. doi:10.1016/j.tetlet.2010.10.084
5. Petchprayoon, C, Yan, Y., Mao, S. and Marriott, G. (2010). Rational design, synthesis and characterization of highly fluorescent optical switches for high-contrast optical lock-in detection (OLID) imaging microscopy in living cells. Bioorganic and Medicinal Chemistry. doi:10.1016/j.bmc.2010.07.015
6. Marriott, G., Mao, S., T. Sakata, Jackson, D. Gomez, T., Aaron, H., Isacoff, EY, Yan, Y. High contrast imaging based on optical lock-in detection imaging of synthetic and genetically encoded optical switches:. PNAS (USA). 105:17789-17794
7. Perrins, RD, Cecere, G., Paterson I. and Marriott, G. (2008). Molecular Dissection and Functional Analysis of Reidispongiolide A binding to actin.Chemistry and Biology, 15, 287-294
8. Mao, S., Benninger, RKW., Piston, D., Jackson, Easley, C., D. Yan, Y. & Marriott, G. (2008). Optical lock-in detection of FRET using genetically encoded optical switches: High contrast FRET imaging of protein interactions in living cells. Biophysical J. 94, 4515–4524
9. Wang, H, Mao, S., Chalovich, J. & Marriott, G. (2008). Tropomyosin dynamics in cardiac thin filaments: A multi-site Foerster resonance energy transfer and anisotropy study. Biophysical J. 94. 4358–4369
